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如何高效率转染人结直肠癌细胞

阅读人数:159 发布时间:2020/6/28 13:53:22

 

西南大学生命科学学院教育部创新重点实验室

使用zeta lifeAdvanced DNA RNA转染试剂

高效率转染HCT-116, SW480, HT-29人结直肠癌细胞

2020-4-28发表文章已见刊

发表文章转染条件

AHCT-116, SW480, HT-29人结直肠癌细胞

BAURKA质粒

C、转染细胞融合度50%

D96孔板每孔使用0.5μg质粒DNA

6孔每孔使用10 ug质粒DNA

(注意:本实验中用到的细胞密度、转染质粒DNA用量不适用于LIPO3000/2000)

发表文章部分内容

Overexpression of AURKA Plasmid for AURKA overexpression was obtained from VectorBuilder. Details about plasmid containing the AURKA gene can be found at https://www.vectorbuilder.cn/vector/VB190802-1063ncc. html. colon cancer cells (HCT-116, SW480, HT-29) were seeded at 50% confluency for 24 h in six-well and 96-well plates. Plasmid (10 ug per-well for six-well and 0.5 μg per-well for 96 plates) and the Advanced DNA RNA transfectionzeta life,USA) reagent were mixed in equal proportionsand added to the cell culture medium for 6 h. DOX (1 μg/ml) was added to induce the expression of AURKA. After addition of DOX and PAL for 36 h, cell viability was measured by MTT and apoptosis was detected by



PAL exerts anti-colon cancer effects by targeting AURKA. (A) PAL promotes apoptosis in colon cancer cells (HCT-116, SW480) in a dose-dependent manner. (B) PAL promotes G2/M phase arrest in colon cancer cells (HCT-116, SW480) in a dose-dependent manner. (C) The expression of AURKA after DOX treatment for 24 h. (D) Cell density after PAL treatment for 24 h in the cells (HCT-116, SW480) transfected with AURKA overexpression plasmids. DOX is an inducer of AURKA

expression in plasmids. (E) Effect of PAL detected by MTT assay on the proliferation ability of colon cancer cells transfected with AURKA overexpression plasmids. (F) Effects of PAL detected by.

zeta lifeAdvanced DNA RNA转染试剂信息如下:

产品名称

货号

规格

Advanced  DNA RNA  转染试剂

AD600025

0.25ML

Advanced  DNA RNA  转染试剂

AD600050

0.5

Advanced  DNA RNA  转染试剂

AD600075

0.75

Advanced  DNA RNA  转染试剂

AD600150

1.5ML

Advanced  DNA RNA  转染试剂

AD600500

5ML

Advanced  DNA RNA  转染试剂

AD601000

10ML

 

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