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美国zeta life胎牛血清成功培养前脂肪细胞(preadipocytes)发表文章已见刊

阅读人数:302 发布时间:2020/5/19 10:27:11

 

一、四川农业大学农场动物遗传资源探索与创新重点实验室,使用美国zeta life胎牛血清成功培养兔前脂肪细胞(preadipocytes)发表文章已见刊;

二、培养条件:

培养细胞名称:兔前脂肪细胞(preadipocytes)

培养体系:6孔板

培养细胞数量:6 × 105

三、用zeta life胎牛血清培养前脂肪细胞(preadipocytes)发表文章部分内容:

Isolation and induction of rabbit preadipocytes Visceral preadipocytes were isolated from perirenal adipose tissue of newborn New Zealand rabbits under sterile condi[1]tions. Briefly, adipose tissues were digested with 0.01% collagenase I (Gibco, Carlsbad, CA, USA). Then, preadipocytes were seeded into 6-well plates at a density of 6 × 105 cells per plate in complete medium (DME/F12, sup[1]plemented with 10% fetal bovine serum [FBS]) (DME/F12 was obtained Gibco, Carlsbad, CA, USA; fetal bovine serumwas from Zeta life, Menlo Park, CA, USA), and preadipocyteswere cultured in a humidified incubator at 37 °C and 5% CO2. Upon reaching confluency (set to day 0), cells were induced by the addition of differentiation medium I (DME/F12 with 1 μM dexamethasone, 500 μM 1-methy1-3-iosbutylxanthine, 1.7 μM insulin, 10% FBS) (dexamethasone, 1-methy1-3- iosbutylxanthine, and insulin were from Solarbio, Beijing, China) for 72 h (day 3). Next, cells were cultured with differ[1]entiation medium II (DME/F12, 1.7 μM insulin, 10% FBS) for an additional 72 h and further cultured in complete medi[1]um until day 9 (day 9). To identify lipid accumulation in cells and obtain mature adipocytes, the accumulation of lipid drop[1]lets was measured by Oil Red O staining. Based on three different phenotypes of lipid accumulation (almost no lipid droplets, a small amount of ring-shaped lipid droplets, and a cluster of bigger lipid droplets), a total of nine cell samples were collected, and each interval time point contained three biological replicates. RT-qPCR was performed to determine the mRNA expression levels of adipogenic marker genes, in[1]cluding PPARG, CEBPA, and FABP4 (primer sequences are shown in Table S1), and the 2-ΔΔCt method was used to ana[1]lyze the relative expression. The housekeeping gene GAPDH served as control.

zeta life胎牛血清信息如下

产品名称

货号

规格

数量

Fetal Bovine Serum France Origin

Z7186FBS-500

500ml

1

Fetal Bovine Serum France Origin 

Z7186FBS-100

100ml

1

Fetal Bovine Serum ,South America Origin

Z7181FBS-500

500ml

1

Fetal Bovine Serum ,South America Origin 

Z7181FBS-100

100ml

1

Fetal Bovine Serum (South America Origin) Ultra-low Endotoxin

Z7180FBS-500

500ml

1

Fetal Bovine Serum (South America Origin) Ultra-low Endotoxin

Z7180FBS-100

100ml

1

Fetal Bovine Serum (Australia Origin

Z7010FBS-500

500ml

1

Fetal Bovine Serum (Australia Origin

Z7010FBS-100

100ml

1

Fetal Bovine Serum ,South America Origin

Z7185FBS-500

500ml

1

Fetal Bovine Serum ,South America Origin

Z7185FBS-100

100ml

1

 

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