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点击次数：1971 发布时间：2022/11/16 14:18:49
线性PEI转染试剂（速溶型）MW40000
英文名Polyethylenimine Linear(PEI) MW40000（rapid lysis）货号KE1098保存条件室温
线性PEI转染试剂是一种阳离子聚合物，分子量40000，它能与核酸形成复合物，并使该复合物进入哺乳动物细胞。线性PEI转染试剂广泛适用于常见细胞系，如 HEK-293、HEK293T、 Hep G2、Hela、CHO-K1、COS-1、COS-7、NIH/3T3 和 Sf9 等。该试剂即使在有血清存在的情况下，它仍然能的将核酸导入细胞。我公司提供的线性PEI转染试剂具有如下优点：高转染效率 , 重组蛋白的高表达水平,与含血清的培养基相兼容, 低细胞毒性,易于操作。
本产品为速溶型，溶解迅速，配制方便。
说明书
Transfection Reagent Preparation ａnd Storage Recommendations
Transfection Reagent Preparation (1 mg/mL)
Materials
1.1g PEI MAX 40K (kingmorn Catalog#KE1098)
2.1L Milli-Q® water, water for injection (WFI), or comparable biological-grade water
3.1N Sodium Hydroxide, USP
4.Sterile 25 mL plastic pipette
5.0.1µm, 0.2µm, or 0.22µm PES vacuum sterile-filter unit
6.Sterile HDPE or polypropylene storage vials
Equipment
1.1L glass beaker
2.PTFE-coated stir bar
3.Magnetic stir plate
4.1L glass graduated cylinder
5.pH meter
6.Pipette controller
7.Vacuum pump with tubing
Method
1.In 1L glass beaker, suspend 1g of PEI MAX 40K in 900 mL water.
2.Add PTFE-coated stir bar to 1L glass beaker ａnd set stirring to produce small vortex.
3.Wait for PEI MAX 40K to completely dissolve. This typically takes less than 5 minutes.
4.Use 25 mL plastic pipette to add 1N sodium hydroxide dropwise to 1L glass beaker until pH is 6.90 to 7.10.
5.If pH accidently exceeds 7.10, use 1N hydrochloric acid ａnd a new 25 mL plastic pipette to lower pH back to 6.90 to 7.10.
6.Decant solution from 1L glass beaker to 1L graduated cylinder.
7.Add water to 1L graduated cylinder to adjust final volume to 1L.
8.Sterile-filter solution through vacuum membrane.
9.Aliquot solution as desired.
10.Store aliquots at 4°C
Transfection Solution Storage ａnd Shelf Life
At 4°C in a suitable container, the transfection solution will retain its maximum performance for at least six months. For qualitative work, the solution may be suitable for use for up to a year.
原创作者：上海创凌生物科技有限公司
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